[INTRODUCTION]
A kind of diagnostic kit to identify the infertile occurred
by anti-immune anti-sperm antibodies, on the basis of
the Mixed Anti-globulin Reaction (MAR) test, which is
quick and intuitionistic diagnostic kit.

[ACTION]
Mixed Anti-globulin Reaction (MAR) test is the standard method recommended by WHO for the detection of anti-sperm antibody (AsAb). The kit, based on classical Coombs Test, is to detect IgG-AsAb (In which case IgA almost does coexist with IgG) by the method of MAR test, and exerts actions of the invitro diagnosis of immunological infertility and the auxiliary diagnosis of seminalduct ligation complication.


[UNITS OF KIT]
a. Solution A 0.5 ml
b. Solution B 0.5ml
c. Solution C 2.5mlX2
d. Slide 10 pieces
e. Coverslip 20 pieces
f. One-off pipette 20 pieces
g. Rubber nipple 2 pieces
h. Instruction 1 sheet

[PROCEDURE]
A. Direct MAR Test
1. After being liquefied at the temperature of 37ºC for 30 minutes, 20 ul of fresh semen is put on slide and mixed with 20 ul of Solution A adequately for 15 seconds.
2. Add 20 ul of Solution B and mix adequately, put a coverlip on the slide. After 3 minutes, observe the results under the Optics Microscope (40 X 10) or Phase Contrast Microscope.
3. Recount after 10 minutes.

B. Indirect MAR Test
1. Centrifuge the sample with negative result in Direct MAR Test.
2. Wash the sample twice with Solution C, then balance the density of spermatozoa to 5X10⁷ /ml.
3. 50 ul of the balanced sperm suspension is added with 200 ul of serum diluted by solution C (1:4), and incubate at the temperature of 37ºC for 1~1.5 hours. The mixture is centrifuged by Solution C in order to remove the dissociative Ig, then balance to 100 ul. The next process and judgment is the same as the Direct MAR Test.

[JUDGEMENT]
If without AsAb on the surface of the spermatozoa, the spermatozoa will swim freely among the adhered sRBC.
If AsAb exist on the surface of the spermatozoa, the sensitized will adhere to the spermatozoa. Under the condition of strong positive, the agglutinates become very massive and the spermatozoa will only twist in the agglutinates marked with sRBC.
At least 100 active spermatozoa should be counted, and calculate the percentage of the active spermatozoa with sRBC compared with the total active spermatozoa.
The Positive Ratio; R = (The quantity of active spermatozoa with sRBC / The quantity of total active spermatozoa) X 100%:
(1) R<10%, Negative
(2) R>10%, Positive
(3) R>40%, Strong Positive. Inferetility caused by AsAb should be considered especially.

[PRECAUTION]
a. Please read this Instruction carefully before using.
b. Fresh semen only.
c. Agitate the Solution A adequately before using.
d. All the solution should be balanced to room Temp., and the crystal in Solution C should be dissolved completely before using.
e. Pipette could be used to smear for twice in order to agitate adequately. The proper area is about 1~2 cm².
f. Adverse influence on the test caused by the temperature should be noticed.

[STORAGE]
Stored at 2~8ºC

[EXPIRY]
12 months 

[LICENSE NUMBER]
GYZZS20030032 http://www.ankebio.com